Application of matrix-assisted laser desorption ionization-time of flight mass spectrometer in identification of clinical common bacteria
10.3969/j.issn.1673-4130.2014.16.047
- VernacularTitle:MALDI-TOF 质谱仪在临床常见细菌鉴定中的应用
- Author:
Bing WANG
;
Xiaoqing REN
;
Meiling CHU
;
Liu YANG
;
Xiangyu SHENG
;
Lianqing ZHOU
;
Wencheng XUE
;
Dongya MENG
- Publication Type:Journal Article
- Keywords:
bacteria;
mass spectrographic analysis;
16S rRNA
- From:
International Journal of Laboratory Medicine
2014;(16):2228-2230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluated the application value of two kinds of mass spectrometer(MS)and Vitek MS system in the i-dentification of routinely isolated bacteria in clinic.Methods 149 strains of common bacteria(including 14 genera and 30 species)i-solated from blood,urine,cerebral spinal fluid,secretion and sputum samples in our hospital from March 2012 to January 2013 were collected and simultaneously identified by 2 kinds of matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALD-TOF-MS).The identification results were compared with those identified by the conventional biochemical identification (Vitek2 compact).The strains with the inconsistent results identified by 3 kinds of method were confirmed by 16S rDNA gene se-quencing.Results Among 149 common bacteria,the correct identification rates of genus and species by the Bruker Biotyper MS were 98% and 96% respectively and which by the Vitek MS system were 97% and 95% respectively.There were no misidentified bacterial strains by these two kinds of MS.Conclusion No statistical difference in the identification results was observed between these two kinds of MS system(P >0.05).Both exhibit excellent identification level and are suitable for the routine laboratory iden-tification of clinical microorganism.
