Expression Changes of Autophagy Associated Protein Beclin-1 and Apoptosis Associated Protein p53 in Rats with Alzheimer's Disease and Regulatory Effects of Electroacupuncture
10.3969/j.issn.1005-5304.2014.09.020
- VernacularTitle:阿尔茨海默病大鼠自噬相关蛋白Beclin-1和凋亡相关蛋白p53表达变化及电针的调控作用
- Author:
Qingchun ZHU
;
Guohong CUI
;
Shuijin SHAO
;
Jinxin TIAN
;
Xiaojing HAN
;
Lisheng ZHANG
;
Pingping LU
;
Haidong GUO
- Publication Type:Journal Article
- Keywords:
electroacupuncture;
AD;
apoptosis;
autophagy;
Beclin-1;
p53;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(9):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of electroacupuncture on the expressions of autophagy related protein Beclin-1 and apoptosis related protein p53 of hippocampus in rats;To explore the mechanism of electroacupuncture on Alzheimer's disease (AD).Methods The rats were randomly divided into the normal group, the sham-operation group, the model group, and the electroacupuncture treatment group. “Baihui” and “Yongquan” points were taken for electroacupuncture treatment and the treatment course was 7 days. The rats were treated once a day for 4 courses. Changes in morphology and number of Nissl positive cells were examined by Nissl staining in hippocampal CA1 regions. Expressions of Beclin-1 and p53 protein were determined by Western blot analysis.Results Number of Nissl positive cells in CA1 region of the model group was significantly less than that of normal group (P<0.01). After electroacupuncture treatment, number of pyramidal cells and expression of Nissl body significantly increased (P<0.05). Expression of Beclin-1 decreased, while expression of p53 increased in the hippocampus of the model group, compared with that in the normal group (P<0.05). However, electroacupuncture treatment could significantly upregulate the expression of Beclin-1 protein (P<0.01), but downregulate the level of p53 (P<0.05).Conclusion Electro-acupuncture treatment could fight against Aβ-induced neuronal apoptosis, and improve the morphological changes of AD’s hippocampus.
