Content Determination of Anthraquinone in Tibet Traditional Medicine Liuwei Nengxiaosan by HPLC
10.3969/j.issn.1005-5304.2014.09.021
- VernacularTitle:HPLC测定藏药六味能消散中蒽醌类成分含量
- Author:
Jincao YANG
;
Jiong CAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Liuwei Nengxiaosan;
aloe emodin;
rhein;
emodin;
chrysophanol;
physcion;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(9):72-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a method for determining the content of anthraquinone in Liuwei Nengxiaosan.Methods The chromatogram column was Eclipse XDB C18 (150 mm×4.6 mm, 5μm), the mobile phase composition was methanol-0.1% orthphosphoric acid (88∶12), the detection wavelength was at 254 nm, the column temperature was 30℃, the flow rate was 1.0 mL/min. Results The linear range of aloe emodin, rhein, emodin, chrysophanol and physcion was 0.076-0.380μg (r=0.999 6), 0.068-0.340μg (r=0.999 8), 0.076-0.380μg (r=0.999 9), 0.070-0.349μg (r=0.999 9), 0.071-0.355μg (r=0.999 7), respectively. The average recovery rate respectively was 97.88% (RSD=0.72%), 97.52% (RSD=0.96%), 98.79% (RSD=0.95%), 97.77% (RSD=1.18%), 96.34% (RSD=2.00%).Conclusion The method is highly effective, quick and sensitive, suitable for quality control of Tibet traditional medicine Liuwei Nengxiaosan.
