Establishment of a miniature pig model of type 1 diabetes by single intravenous injection of high dose streptozotocin
10.3969/j.issn.1005-4847.2014.04.003
- VernacularTitle:一次性静脉注射高剂量链脲佐菌素建立1型糖尿病小型猪模型
- Author:
Yongchun CUI
;
Kai LI
;
Hong ZHANG
;
Xiaopeng LIU
;
Weimin YUAN
;
Jubo LI
;
Aili WU
;
Yue TANG
- Publication Type:Journal Article
- Keywords:
Type 1 diabetes;
Mini-pig;
Streptozotocin;
Blood glucose
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(4):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of establishing mini-pig model of type 1 diabetes by single intrave-nous injection of high dose streptozotocin .Methods Eight male Zhonghua mini-pigs (22.6 ±1.8 kg) were administrated with high-dose streptozotocin (150 mg/kg) into the ear vein .Before and 10, 30, 90 minutes, 1, 3 and 7 days after adminis-tration of streptozotocin , blood samples were obtained respectively , and used to dynamically monitor the fasting blood-glucose. C peptide and insulin levels were evaluated by IVGTT test .Results Since 24 hours after STZ administration , the fasting blood-glucose level was increased significantly compared with that of pre-administration and maintained at 16.7-20.6 mmol/L, while the C peptide and insulin levels were decreased significantly .IVGTT results showed that blood sugar levels at 1 h af-ter intravenous injection of 50%glucose were much higher than 11.1 mmol/L and failed to restore to fasting glucose levels until 2 h, insulin and c-peptide did no response after injection of glucose , always kept at a trace level .Conclusion A sin-gle high-dose streptozotocin injection can be used to establish a mini-pig model of type 1 diabetes successfully .
