Immunogenicity of a vaccine of S fragments from a new type reovirus generates humoral and cellular immune responses in mice
10.3969/j.issn.1005-4847.2014.04.006
- VernacularTitle:新型呼肠病毒 S基因 DNA 疫苗在小鼠体内的免疫原性
- Author:
Jun HOU
;
Ze LIU
;
Guoming XIE
;
Shengdong LUO
;
Ruisheng LI
;
Bingke BAI
- Publication Type:Journal Article
- Keywords:
Reovirus;
DNA vaccine;
Cellular immunity;
Humoral immunity;
Mouse
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(4):24-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the humoral and cellular immune responses of a vaccine of S fragments from a new type reovirus R4 strain in mice.Methods Four recombinant plasmids were constructed by respectively cloning S 1, S2, S3,S4 genes into pcDNA3.1+, and mice were intramuscularly immunized with the recombinant plasmids in a dose of 100 μg/mouse.Control vector pcDNA3.1 +and phosphate buffered saline (PBS) were used as negative controls.The spe-cific antibody level and IgG subclass (IgG1, IgG2a, and IgG2b) were detected by ELISA, and cellular immune responses to R4 were assessed using an interferon ( IFN)-γELISpot assay .Results All recombinant plasmids induced significantly higher levels of anti-R4 IgG compared with that of the controls (pcDNA3.1 +and PBS), and the titers were highest in the mice immunized with S1 and S3.On the other hand, S1 gene induced highest IgG2a antibody and the cellular immune re-sponse was best .Conclusions After the mice immunized with S 1 gene recombinant plasmid , this plasmid can initiate both cellular and humoral immune responses in mice .S1 gene recombinant plasmid is a promising vaccine candidate .
