Establishment of adipocytokine CTRP4 transgenic mouse
10.3969.j.issn.1671.7856.2014.007.001
- VernacularTitle:脂肪细胞因子 CTRP4转基因鼠的构建与鉴定
- Author:
Daxiang NA
;
Zhuang MA
;
Yang LUO
;
Qi LI
;
Weifeng TAN
;
Lanlan WANG
;
Guoying ZHANG
;
Ang YIN
;
He HUANG
;
Xiaotong WU
;
Lu WANG
- Publication Type:Journal Article
- Keywords:
CTRP4;
Transgenic mice;
Adipocytokine
- From:
Chinese Journal of Comparative Medicine
2014;(7):1-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the CTRP4 transgenic mouse model and investigate the function of the novel adipocytokine CTRP4.Methods CTRP4 overexpressing vector in pCAGGS was firstly constructed and then microinjected into zygote to establish the founder transgenic mice .F1 heterozygotes were generated by founder mice mating with wildtype mice, and the CTRP4 transgenic homozygotes were generated by F 1 littermates.The genotype was confirmed by PCR and test cross method .The expression level of CTRP 4 in transgenic mice was detected by western blot .Result The human CTRP4 transgenic homozygote mice line was established , and the expression level of CTRP 4 was confirmed raletively high in detected tissues including heart , liver, brain and kidney . Conclusion The human CTRP4 transgenic mice was successfully established .
