In vivo imaging of 99 m Tc-Glucarate-labeled acute necrotic myocardium using microSPECT/CT
10.3969.j.issn.1671.7856.2014.007.003
- VernacularTitle:利用小动物 SPECT／CT 和99m锝-葡糖二酸评估急性缺血坏死心肌动物
- Author:
Luer BAO
;
Lin ZHANG
;
Xiaohui ZHOU
;
Qingshu MENG
;
Zhenzhen ZHAN
;
Huimin FAN
- Publication Type:Journal Article
- Keywords:
Rats;
Ischemic-reperfusion injury;
99m Tc;
Glucarate;
microSPECT/CT
- From:
Chinese Journal of Comparative Medicine
2014;(7):14-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anatomic localization and size of acute necrotic myocardium in the ischemic-reperfused rat hearts using 99m TC-Glucarate and microSPECT/CT.Methods The ischemic-reperfused ( IR) rat heart models were established by ligating left anterior descending coronary artery for 60 min.99mTC-Glucarate was intravenously injected into the rats 24 hours after IR operations .Images were acquired 30 min after administration of 99m TC-Glucarate using microSPECT/CT. Anatomic localization and size of acute necrotic myocardium were analyzed with microSPECT/CT imaging , and these results were compared to those determined by triphenyltetrazolium chloride ( TTC ) staining.Results The microSPECT/CT images showed hot spot accumulations of 99mTC-Glucarate in IR hearts (the heart-to-liver ratio was 1.90 ±0.33), not in controls (P <0.05).The anatomic localization of 99mTC-Glucarate-labeled necrotic myocardium were in correspondence with TTC staining results .The hot spot size was related significantly to necrotic myocardial size determined by TTC staining ( R2 =0.964 ) .Conclusions The localization and size of acute necrotic myocardium can be assessed by non-invasive microSPECT/CT imaging with99m Tc-Glucarate.
