In vitro cytotoxicity of the hydroxyapatite/barium titanate biological piezoelectric ceramic coating
10.3969/j.issn.2095-4344.2014.34.010
- VernacularTitle:羟基磷灰石/钛酸钡压电陶瓷涂层的体外细胞毒性
- Author:
Wen KANG
;
Guoqiang XU
;
Aji DILNUR
;
Qng WEI
;
Hui MA
;
Zhaopeng LI
;
Qian SONG
;
Zulati RUFIYA
;
Abdujilil ALINA
;
Kaisar PAHIRDIN
- Publication Type:Journal Article
- Keywords:
ceramics;
materials testing;
hydroxyapatites
- From:
Chinese Journal of Tissue Engineering Research
2014;(34):5466-5472
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In order to optimize the biological activity of hydroxyapatite, previous experiments have used plasma spraying technique to prepare a piezoelectric ceramic coating on the surface of hydroxyapatite, but the cytotoxicity of this new material is not clear.
OBJECTIVE: To evaluate the cytotoxicity of hydroxyapatite/barium titanate biological piezoelectric ceramic coatingin vitro.
METHODS: The 3rd generation beagle bone marrow mesenchymal stem cels were seeded on
hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens, respectively.
After 5 days, the celladhesion was detected by scanning electron microscopy. The 3rd generation bone marrow
mesenchymal stem cels were also co-cultured with hydroxyapatite/barium titanate piezoelectric ceramic specimen extract, hydroxyapatite specimen extract, low-glucose Dulbecco’s modified Eagle’s medium containing 5%
dimethylsulfoxide and 15% fetal bovine serum, and low-glucose Dulbecco’s modified Eagle’s medium containing 15%
fetal bovine serum, respectively. The cytotoxicity was tested by cellCounting Kit-8 assay at days 1, 3, 5 after co-culture. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cels on the surface of hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens grew proliferatively and presented with multi-layer
growth. The connection between cels and pseudopodia was very close, which indicates that the two kinds of materials both have good cytocompatibility. cellCounting Kit-8 assay showed that the cels cultured in the extracts of
hydroxyapatite/barium titanate biological piezoelectric ceramic and hydroxyapatite specimens proliferated more than 80%, and the toxicity was grade 1 that meant no cytotoxicity.
