A method for determination of trace content of protein in material of chitin/chitosan
- Author:
Tu Thi Ngoc Nguyen
- Publication Type:Journal Article
- Keywords:
chitin
- MeSH:
Chitin;
Chitosan;
Proteins
- From:Pharmaceutical Journal
2002;319(11):23-24
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Protein trace present in chitin/chitosan product has been determined by our proposed method in which protein trace included in chitin/chitosan was extracted by refraction in 10% NaOH solution at 100oC for 5 hours. Chitin/Chitosan precipitate was discarded. protein trace in the solution was restored by neutralizing with HCl 10N to get the yellow powder. This powder was then analyzed for total nitrogen content by Kjeldahl method. The control method was conducted at the same procedure excepted from the presence of 10% NaOH. The results of different chitin/chitosan samples obtained from these two methods showed that amount of protein trace obtained from above proposed method was somewhat higher than that of the control one. Besides, the purified chitosan sample gave the lower protein trace content than that of the technical chitosan sample as well as chitin one. Protein trace bound between macromolecules of chitin/chitosan was separated in the proposed method by boiling water under alkaline condition whereas it was not completely separated in the control method because sodium hydroxide was absent.
