DNA sequencing of HEV ORF3 cDNA and expression in HepG2 cell
10.3969/j.issn.1671-8348.2014.23.021
- VernacularTitle:HEV ORF3 cDNA 序列分析及在 HepG2细胞的表达
- Author:
Zhenyu ZHAO
;
Wenjing WEN
;
Xinsheng LIU
;
Jiangbo ZHAO
;
Xiaodong LIU
- Publication Type:Journal Article
- Keywords:
hepatitis E virus(HEV);
open reading frame(ORF);
expression vector
- From:
Chongqing Medicine
2014;(23):3034-3036
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analysis the DNA sequence of HEV ORF3 cDNA and express in HepG2 cell .Methods Recombinant vector carrying HEV ORF3 cDNA was constructed and identified by DNA sequencing .Expression of recombinant vector in HepG2 cell was determined by immunofluorescence technique .Results The recombinant vector was constructed and expressed in HepG2 cell successfully .Conclusion The research laid experimental foundation for exploring the biological function of ORF3 protein in the pathogenesis of viral hepatitis E .
