Biocompatibility of Genipin cross-linked type I collagen with human adipose-derived stem cells in vitro
10.3969/j.issn.2095-4344.2014.34.003
- VernacularTitle:京尼平交联Ⅰ型胶原蛋白材料与人脂肪间充质干细胞的生物相容性
- Author:
Gang WANG
;
Ting KANG
;
Yi LIU
;
Gangqiang LIU
- Publication Type:Journal Article
- Keywords:
adipose tissue;
mesenchymal stem cels;
colagen type I;
cross-linking reagents;
biocompatible materials
- From:
Chinese Journal of Tissue Engineering Research
2014;(34):5423-5428
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Low toxicity of Genipin has certain species and cellspecificity. Biocompatibility of Genipin cross-linked type I colagen with human adipose-derived stem cels is essential for construction of
tissue-engineered adipose.
OBJECTIVE:To investigate the bbiocompatibility of Genipin cross-linked type I colagen with human adipose-derived stem cels.
METHODS:Human adipose-derived stem cels were isolated and cultured to the third generation, and the cels were seeded on Genipin cross-linked type I colagen scaffold. MTT assay was used to evaluate the adhesion and proliferation of cels on the scaffold, and the toxic effects of Genipin cross-linked type I colagen on human
adipose-derived stem cels. Optical microscopy and scanning electron microscopy were utilized to observe the adhesion and growth process of human adipose-derived stem cels on the scaffold as wel as the morphological changes of cels.
RESULTS AND CONCLUSION:Human adipose-derived stem cels could adhere to the scaffold immediately
after seeded and increase gradualy on the scaffold, with the average adhesion rate of 86.5%. Optical microscopy and scanning electron microscopy showed that human adipose-derived stem cels adhered wel on the scaffold. The cels increased gradualy over time, and could migrate into the scaffold, and distribute evenly with the passage of time when observed with optical microscopy. The result showed Genipin possesses very low cytotoxicity to the cels, and the outstanding biocompatibility is found between the cels and scaffoldin vitro after cross-linked with Genipin.
