Effects of silencing STMN1 by siRNA on sensitivity of oesophageal cancer cells Eca-109 to paclitaxel
10.3969/j.issn.1671-8348.2014.22.001
- VernacularTitle:siRNA沉默STMN1对食管癌 Eca-109细胞紫杉醇敏感性的影响
- Author:
Hongwu ZHU
;
Dan JIANG
;
Ziying XIE
;
Meihua ZHOU
;
Dayong SUN
;
Yagang ZHAO
- Publication Type:Journal Article
- Keywords:
STMN1;
siRNA;
oesophageal cancer;
paclitaxel;
chemosensitivity
- From:
Chongqing Medicine
2014;(22):2833-2835
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of silencing STMN1 by siRNA on the sensitivity of oesophageal cancer cells Eca-109 to paclitaxel .Methods The STMN1 siRNA(siSTMN1) or scramble siRNA(SCR) were transient transfected into Eca-109 cells .The mRNA and protein levels of STMN1 were detected by qPCR and Western blot in the Eca-109 cells of different groups .In vitro paclitaxel sensitivity of siSTMN1 and SCR transfected Eca-109 cell lines was tested by MTT assay and colony formation as-say .Hoechst 33258 nuclear staining were used to investigate the effect of silencing STMN 1 on the sensitivity of SCR ,siSTMN1 transfected Eca-109 cells and nontreated counterparts under paclitaxel induced apoptosis .Results The transient transfection cell lines were successfully established .Both protein and mRNA levels of STMN1 were effectively down-regulated in the siSTMN1 transfected Eca-109 cells .Down-regulation of STMN1 significantly enhanced the sensitivity of Eca-109 cells in response to paclitaxel (P<0 .01) .In addition ,the siSTMN1 transfected Eca-109 cells displayed significant apoptosis as assessed by Hoechst nuclear stai-ning(P<0 .01) .Conclusion Silencing STMN1 by siRNA could enhance the sensitivity of oesophageal cancer cells Eca-109 to paclitaxel .
