The role of suppression of p38 MAPK in cellular vacuole formation
10.3969/j.issn.1671-8348.2014.22.004
- VernacularTitle:阻断p38丝裂原活化蛋白激酶在细胞空泡形成中的作用
- Author:
Chunyan ZHANG
;
Chunhong FENG
;
Jianxiong JING
;
Chunyan DUAN
;
Youping LIU
;
Xianming XIA
;
Hong LI
;
Rongyang DAI
;
Shaokun CHEN
- Publication Type:Journal Article
- Keywords:
cytoplasmic vacuoles;
p38 MAPK;
endoplasmic reticulum;
SB203580
- From:
Chongqing Medicine
2014;(22):2841-2844
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of the p38 MAPK pathway in the formation of cytoplasmic vacuoles .Methods Af-ter treated with Anisomycin ,SB203580 or SP600125 ,images of HepG2 ,LM3 ,QBC939 ,Hela and A549 cells were recorded by light microscopy and taken at a magnification of 400 × .The effects of anisomycin ,SB203580 and SP600125 on the activity of p38 and JNK were measured by Western blot .LM3 and A549 cells were stained with the ER-tracker red and the lyso-tracker red and subjec-ted to confocal microscopy analysis .Results (1)Anisomycin could abolish cytoplasmic vacuolization of HepG2 cells .(2)p38 MAPK activation was responsible for anisomycin-induced cytoplasmic vacuolization abolishment .(3)p38 MAPK blocking initiated cytoplas-mic vacuoles formation in various cancer cell lines .(4)p38 MAPK blocking-induced cytoplasmic vacuoles disrupted the integrity of endoplasmic reticulum .(5)p38 MAPK blocking reversibly induced cytoplasmic vacuoles formation .Conclusion These observations provide direct evidence for a role of p38 MAPK signaling in regulating the formation of cytoplasmic vacuoles .
