Effects of hypertonic saline on TNF-α、IL-1β、S-100β following focal cerebral ischemia-reperfusion injury in rats
10.3969/j.issn.1006-5725.2014.16.003
- VernacularTitle:高张盐水后处理对大鼠局灶性脑缺血再灌注损伤TNF-α、IL-1β、S-100β的影响
- Author:
Yongdong LAI
;
Wenxiang JIN
;
Rongzhi HE
- Publication Type:Journal Article
- Keywords:
Isehemia-reperfusion;
Hypertonic saline;
TNF-α;
IL-1β;
S-100β
- From:
The Journal of Practical Medicine
2014;(16):2532-2534
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hypertonic saline on TNF-а, IL-1β, S-100β level following focal cerebral ischemia-reperfusion injury in rats and explore the mechanisms involved. Methods Ninety rats were randomized equally into 4 groups,namely the pseudo-operated group (A group),untreated IR injury group (B group),C group (4.2% hypertonic saline) and D group (7.5% hypertonic saline). Cerebral ischemia was induced by middle cerebral artery occlusion for 2 h followed by administration of the corresponding treatments in group C and D. After 22 h of reperfusion , TNF-а, IL-1β, S-100βexpression in the ischemic brain tissue were measured by enzyme-linked immunosorbent assay (ELISA). Results After hypcrtonic saline treatment, the two saline-treated groups showed significant reduction in TNF-а、IL-1β、S-100β levels , but were still higher than pseudo-operated group(A group). Compared with B group, significant difference can be seen among C and D group. Conclusion Hypcrtonic saline can reduce cerebral TNF-а、IL-1β、S-100βlevel of isehemia-reperfusion injury.
