The influence of repetitive transcranial magnetic stimulation on learning, memory and the ultrastructure of the CA3 region of the hippocampus
10.3760/cma.j.issn.0254-1424.2014.07.002
- VernacularTitle:重复经颅磁刺激对抑郁大鼠学习记忆及海马CA3区超微结构的影响
- Author:
Bing BAI
;
Lin ZHAO
;
Zhaohui ZHANG
- Publication Type:Journal Article
- Keywords:
Depression;
Repetitive transcranial magnetic stimulation;
Learning;
Memory;
Hippocampus
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2014;36(7):503-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of repetitive transcranial stimulation (rTMS) on learning and memory and on the neuron and synapse ultrastructures of the CA3 region of the hippocampus.Methods Forty Sprague-Dawley (SD) rats were randomly divided into 4 groups (n =l0 in each group):a normal control group,a depression group,an rTMS group and a sham group.Unpredictable mild stress was used to establish depression models in the rats of the latter3 groups.The sucrose water consumption test and open-field test were used to evaluate any depressive behavior of each group.The rTMS group rats were given 15 Hz rTMS for 21 days while the sham group received sham stimulation.The orientational navigation and spatial probe tests were performed on each group using a Morris water maze to evaluate their learning and memory abilities.In addition,changes in the ultrastructure of the CA3 region of the hippocampus were detected using transmission electron microscopy.Results The modelling induced significant differences in the sucrose water consumption test results and in horizontal and vertical behavior in the open-field tests.Escape latency and spatial probe time were significantly different between the rTMS group and the sham and depression groups.There was no significant difference in the behavioral indexes between the depression group and the sham group.Electron microscopy showed pathological changes in the ultrastructures of the neurons and synapses in the CA3 region of the hippocampus among the depression group,while in the rTMS group those ultrastructures tended to be basically normal.Conclusion rTMS can improve learning and memory during depression,at least in rats.A possible mechanism is that rTMS can induce changes in the ultrastructures of neurons and synapses in the CA3 region of the hippocampus.
