Effects of long-term intraperitoneal flurbiprofen axetil on gastric mucosa and liver function in rats with bone cancer pain
10.3760/cma.j.issn.0254-1416.2014.06.017
- VernacularTitle:长期腹腔注射氟比洛芬酯对骨癌痛大鼠胃粘膜及肝功能的影响
- Author:
Zhengquan HU
;
Yu ZHOU
;
Zhengliang MA
;
Xiaoping GU
- Publication Type:Journal Article
- Keywords:
Flurbiprofen;
Bone neoplasms;
Pain;
Gastric mucosa;
Liver function tests
- From:
Chinese Journal of Anesthesiology
2014;34(6):708-711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of long-term intraperitoneal flurbiprofen axetil on the gastric mucosa and liver function in rats with bone cancer pain (BCP).Methods Thirty-six healthy female Sprague Drawley rats,weighing 200-250 g,were randomly divided into 6 groups (n =6 each):BCP + normal saline group (group BCP),BCP + flurbiprofen axetil 10 mg·kg-1 ·d-1 group (group BF10),BCP + flurbiprofen axetil 25 mg· kg-1 · d-1 group (group BF25),BCP + flurbiprofen axetil 50 mg· kg-1 · d-1 group (group BF50),flurbiprofen axetil 50 mg·kg-1 ·d-1 group (group F50),and sham operation + normal saline group (group S).BCP was induced by injecting Walker 256 mammary gland cancer cells into the bone marrow of the left tibia.In BCP and S groups,paw withdrawal threshold to mechanical stimulation (MWT) was measured at 1 day before inoculation of cancer cells (T0) and 3,5,7,10,14,17 and 21 days after inoculation (T1-7).The corresponding doses of flurbiprofen axetil or normal saline were administered intraperitoneally twice a day at 12 h intervals starting from T7 for 7 consecutive days.Blood samples were obtained from the abdominal aorta to determine the levels of alanine transaminase (ALT),aspartate transaminase (AST),total protein (TP),and albumin (ALB) in serum.The rats were then sacrificed and stomach and livers were removed for microscopic examination of pathological changes.The ulcer index (UI) was calculated.Results Compared with group S,MWT was significantly decreased at T2-7,no significant changes were found in the other parameters mentioned above in BCP group,the serum levels of TP and ALB were decreased in BF50 and F50 groups,and UI was increased in BF25,BF50 and F50 groups.Compared with BCP group,the serum levels of TP and ALB were decreased in BF50 and F50 groups,and UI was increased in BF25,BF50 and F50 groups.No significant changes were found in each parameter between BF50 and F50 groups.Microscopic examination showed that the epithelium of gastric mucous membrane became defect in partial region of the stomach and showed damage to gastric glands in the deep part and massive inflammatory cell infiltration in BF50 and F50 groups.Microscopic examination showed mild damage to the integrity and continuity of gastric mucosa and did not show any abnormality in livers in BF25 group.Conclusion Long-term intraperitoneal flurbiprofen axetil can cause damage to the gastric mucosa dose-dependently and has no effect on liver function in rats with BCP.
