Effect of ixeris sonchifolia on mitochondrial transcription factor A expression following myocardial injury induced by exhausting exercise in rats
10.3760/cma.j.issn.0254-1416.2014.07.027
- VernacularTitle:苦碟子对力竭运动致大鼠心肌损伤时线粒体转录因子A表达的影响
- Author:
Shuang HAN
;
Kun HE
;
Yadong ZHAO
;
Junfang RONG
- Publication Type:Journal Article
- Keywords:
Asteraceae;
Hyperkinesis;
Cardiomyopathies;
Mitochondria;
Transcription factors
- From:
Chinese Journal of Anesthesiology
2014;34(7):876-878
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of ixeris sonchifolia on mitochondrial transcription factor A (mtTFA) expression following myocardial injury induced by exhausting exercise in rats.Methods Forty male Wistar rats,weighing 200-220 g,were randomly divided into 3 groups using a random number table:control group (group C,n =8),exhausting exercise group (group ES,n =16) and ixeris sonchifolia group (group IS,n =16).The model of myocardial injury was established by exhausting swimming.In IS group,the rats were subjected to exhausting swimming after intraperitoneal ixeris sonchifolia 20 g/kg.In ES and IS groups,8 rats were chosen at 6 and 24 h after exhaustion,and blood samples were taken from the inferior vena cava for determination of serum cardiac troponin I (cTnI) concentrations by ELISA.The animals were sacrificed after blood sampling and myocardial specimens were obtained for microscopic examination and for determination of mtTFA expression by Western blot.Results Compared with group C,the concentration of serum cTnI was significantly increased,and the expression of mtTFA in myocardial tissues was down-regulated at 6 and 24 h after exhaustion in ES and IS groups.Compared with group ES,the concentration of serum cTnI was significantly decreased,and the expression of mtTFA in myocardial tissues was up-regulated at 6 and 24 h after exhaustion in group IS.Conclusion Ixeris sonchifolia can reduce exhausting exercise-induced myocardial injury through up-regulating myocardial mtTFA expression in rats.
