Role of oxidative stress in spinal neurotoxicity induced by ropivacaine in rats
10.3760/cma.j.issn.0254-1416.2014.07.016
- VernacularTitle:氧化应激在罗哌卡因诱发大鼠脊髓神经毒性中的作用
- Author:
Xiaoping XU
;
Zhihua SUN
;
Qulian GUO
- Publication Type:Journal Article
- Keywords:
Oxidative stress;
Amides;
Spinal cord;
Drug toxicity
- From:
Chinese Journal of Anesthesiology
2014;34(7):833-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of oxidative stress in the spinal neurotoxicity induced by ropivacaine in rats.Methods Ninety pathogen-free male Sprague-Dawley rats,weighing 250-320 g,aged 3 months,in which intrathecal catheter was successfully implanted into L5,6 interspace without complications,were randomly divided into 3 groups (n =30 each) using a random number table:control group (group C),ropivacaine group (group R),and antioxidant Tempol group (group T).The rats received 1％ ropivacaine 1.2 μg/g for 8 times at 1.5 h intervals through the catheter in R and T groups,while the rats received the equal volume of normal saline instead in group C.In T group,Tempol 20 μg/g was injected intrathecally at 4,8,12,24,48 and 72 h after the last injection of ropivacaine.The rats were sacrificed at 1,3,5,7 and 14 days after the end of ropivacaine injection,and their lumbar enlargements were removed for TUNEL staining to detect the cell apoptosis.SOD activity was determined by colorimetry and MDA content was measured using TBA photoelectric colorimetry.Apoptosis index was calculated.Results Compared with group C,apoptosis index and MDA content were significantly increased,and SOD activity was decreased in R and T groups.Compared with group R,apoptosis index and MDA content were significantly decreased,and SOD activity was increased in group T.Conclusion Oxidative stress is involved in the development of spinal neurotoxicity induced by ropivacaine in rats.
