Experimental studies of the correlation between vitronectin and collagen expressions post-irradiation
10.3760/cma.j.issn.0254-5098.2014.08.004
- VernacularTitle:辐射后玻连蛋白与胶原蛋白表达相关性的实验研究
- Author:
Mengchen XUE
;
Xuwei CAI
;
Qi LIU
;
Qin ZHANG
;
Congying XIE
;
Xiaolong FU
- Publication Type:Journal Article
- Keywords:
Vitronectin;
Collagen;
Biological Markers;
Radiation;
Fibroblast
- From:
Chinese Journal of Radiological Medicine and Protection
2014;34(8):573-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze radiation induced alterations of vitronectin and collagen expressions in fibroblasts at different times post-irradiation,so as to evaluate the potential to apply vitronectin as a biomarker of radiation-induced lung fibrosis.Methods The human fibroblast cells WI-38 and IMR-90 were irradiated with 137Cs γ-rays at doses of 0 (control),4,6,8,10 and 12 Gy,respectively.The cells and its supernatant were collected at 6,12,24,36,48 and 60 h post-irradiation.The expressions of vitronectin and collagen Ⅰ and Ⅲ were analyzed by Western blot,PCR and ELISA.Results After irradiation,the expressions of vitronectin and collagen Ⅰ and Ⅲ were positively correlated (r=0.40-0.79,P＜0.05) and were all significantly higher than that in control group (t =3.04-25.45,P ＜0.05) and reached the highest expression levels at 48 h after 8-10 Gy of irradiation (t =2.92-18.86,P ＜ 0.05).Analyses of Real-time PCR and ELISA assay showed that expressions of vitronectin mRNA and its protein level in the cell lysis were significantly increased by radiation (F =27.09-42.62,P ＜ 0.05).Conclusions The expressions of vitronectin in cellular supernatant and its mRNA may be a potential biomarker of radiation-induced fibrosis,and 48 h after 8 Gy irradiation may be an optimum condition of measurement.
