Effects of glucose metabolic disorder on adipose-derived cytokine and cognitive impairment in olanzapine-induced obese rats
10.3760/cma.j.issn.1674-6554.2014.07.005
- VernacularTitle:奥氮平诱导肥胖大鼠糖脂代谢紊乱对细胞因子和认知功能损伤的影响
- Author:
Xiaojie MA
;
Wenhui ZHANG
;
Xiaolong XIA
;
Xueping GUI
- Publication Type:Journal Article
- Keywords:
Olanzapine;
Obese rat;
Adipose-derived cytokine;
Cognitive impairment
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2014;23(7):591-593
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the adipose-derived cytokine changes and aggravate cognitive impairment in olanzapine-induced obese rats caused by glucose metabolic disorder.Methods 20 rats fed with ordinary fodder were used as normal control group,olanzapine group of 20 rats fed with olanzapine(1.2 mg · kg-1) and ordinary fodder for 4 weeks.Successfully established experimental model rats induced by olanzapine after 4 weeks.Serum tumor necrosis factor α(TNF-α),interleukins 6 (IL-6) and C-reactive protein (CRP) contents were measured by Elisa.Serum glucose contents were determined by biochemical colorimetric method and blood lipid contents determined with automatic biochemical analyzer.Learning,memory capacity and escape latency were detected with Maze test.Results After administration 4 weeks,the levels of body weight,blood glucose and blood lipid in olanzapine group were higher than those in control group.The serum TNF-α((1.57±0.04) ng/ml),IL-6((127.47±11.38) pg/ml) and CRP ((2.68±0.06) mg/ml) in olanzapine group rised,compared with control group ((0.59±0.03) ng/ml,(96.58± 8.77) pg/ml and (1.86±0.04) mg/ml respectively),the differences were statistically significant(P＜0.05).Electric shocks and escape latency in olanzapine group were higher than those in control group (P＜0.05).The FBS had positive correlation with hs-CRP,IL-6 and TNF-α respectively (r=0.385,0.260,1.280; all P＜0.05).Conclusion Olanzapine can induce metabolic disturbance of blood glucose,blood hpid,and the increase of serum TNF-α,IL-6 and CRP levels in rats.Positive correlation is showed between TNF-of and FBS.Hyperglycemia can promote cell toxicity and leads to cognitive dysfunction in rats.
