Screening and identification of the colonic adaptation-related proteins in ultra-short bowel rats

VernacularTitle:超短肠大鼠结肠代偿相关蛋白筛选与鉴定
Author: Jun HU ; Shaoyuan LI ; Junfeng LUO ; Yuliang LYU ; Xuewu PAN ; Lei SUN
Publication Type:Journal Article
Keywords: Colonic adaptation; Proteomics; Rats; Ultra-short bowel
From: Chinese Journal of Clinical Nutrition 2014;22(4):239-243
CountryChina
Language:Chinese
Abstract: Objective To screen and identify the colonic adaptation-related proteins.Methods Totally 20 SD rats were equally randomized into 2 groups:ultra-short bowel group (90％ of intestine was resected) and control group (transection and then anastomosis of the intestine).After 21 days of feeding,two-dimensional electrophoresis (2-DE) was performed to separate the total protein from the colon mucous epithelial tissues.Then Image Master 2D Platinum software was use to analyze differential expressional proteins,which were further analyzed by MALDI-TOF-MS and Mascot software to identify these proteins.Results The 2-DE patterns with high resolution were obtained from the colon mucous epithelial tissues of both groups.Four differentially expressed proteins were identified by MALDI-TOF-MS:M2-PK,AGR2,proteasome activator complex subunit 1,and pancreatic triacylglycerol lipase.These proteins were involved in sugar and fat metabolism and cell proliferation.Conclusion The identified proteins may play important roles in the process of colonic adaptation through substance metabolism and by stimulating cell proliferation.