Correlations among different measurement indicators of intestinal barrier
10.3760/cma.j.issn.1674-635X.2014.04.005
- VernacularTitle:肠道屏障功能测定方法的相关性
- Author:
Jie WANG
;
Guizhen HE
;
Yukang WANG
- Publication Type:Journal Article
- Keywords:
Intestinal barrier function;
Diamine oxidase;
Endotoxin;
Glutamine;
Lactulose/mannitol;
Correlation
- From:
Chinese Journal of Clinical Nutrition
2014;22(4):213-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study several measuring methods of the intestinal mucosa barrier and evaluate the correlation between different methods for determining the intestinal damage.Methods Sixteen specific pathogen free (SPF) male Sprague-Dawley rats were randomly divided into two groups:the Control group (n =8) and the ischemia/reperfusion (I/R) group (n =8).After adaptive feeding for 5 days,I/R group was put into ischemia model for 60 min and the Control group was merely opened on its abdominal wall but without ischemia for 60 min.After having been fed for another day,all rats were killed and specimens were collected.The plasma diamine oxidase (DAO),D-lactate (D-LAC),endotoxin,and glutamine (Gln) levels were detected,and the intestinal mucosal morphology was observed.The intestinal permeability (L/M) was detected 1 day before and after the surgery.Results The plasma DAO,D-LAC,and endotoxin levels were significantly higher in I/R group compared with the Control group (DAO:(0.498 ±0.032) vs (0.247 ±0.051) U/ml,t=-11.790,P=0.000; D-LAC:(5.47±1.55) vs (3.83±0.63) mg/L,t=-2.757,P=0.022; endotoxin:(0.0395±0.002 8) vs (0.025 6 ±0.004 5) EU/ml,t =-7.377,P =0.000).The plasma Gln concentration was significantly lower than that in the Control group [(646.12 ± 34.75) vs (839.13 ± 163.76) μmol/L,t =3.261,P =0.012).The L/M value on the 1 st postoperative day was significantly higher than that in the I/R group [(3.63 ±2.09) vs (1.22 ±0.66),t =-3.118,P =0.013)].The jejunum mucosal thickness,jejunum villus height,ileal mucosal thickness,and ileal villus height were significantly lower in I/R group compared with the Control group after operation [(329.80 ±64.68) vs (512.82 ±38.41) μm,t=6.881,P=0.000; (253.06±69.33) vs (386.79±56.39) μm,t=4.232,P=0.001; (205.89± 18.71) vs (335.29±27.71) μm,t=10.945,P=0.000; (135.61 ±22.30) vs (253.18±31.02) μm,t =8.705,P =0.000].After intestinal ischemia/reperfusion,DAO,D-LAC,endotoxin and L/M were all increased and positively correlated with each other.The plasma concentration of Gln and the morphological changes of jejunum and ileum were negatively correlated with DAO,D-LAC,endotoxin,and L/M,respectively.Conclusions After intestinal ischemia/rcperfusion,the levels of all examination indicators obviously change and correlate with each other.The DAO,D-LAC,endotoxin,and L/M are positively related to each other and negatively correlated with the intestinal barrier function.Gln is positively correlated with small intestinal mucosal morphology and negatively correlated with others,respectively.
