The protective of folic acid supplementation in different ways on lipopolysaccharide-induced teratogenesis in mice
10.3760/cma.j.issn.1672-7088.2014.22.001
- VernacularTitle:不同方式补充叶酸对细菌脂多糖诱发胎鼠畸形的预防效应
- Author:
Jun ZHOU
;
Mei ZHAO
;
Yuanhua CHEN
;
Shuxian WU
;
Xiaoling YU
;
Jianhong LIAO
;
Dexiang XU
- Publication Type:Journal Article
- Keywords:
Folic acid;
External malformations;
Skeletal malformations
- From:
Chinese Journal of Practical Nursing
2014;30(22):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of folic acid supplementation in different ways on lipopolysaccharide-induced external malformations and skeletal malformations in mice.Methods The pregnant mice were divided into six groups randomly,including saline control group,FA control group,LPS group,LPS+FAig group,LPS+FAip group,and LPS+FAdw group.All dams were medicated on gestational day 8～12,and then sacrificed on gestational day 18,for each litter,the number of live fetuses,dead fetuses,resorption fetuses and external malformations of live fetuses were all counted.All fetuses were subsequently evaluated the skeletal malformations.Results A low dose of LPS injection during the second trimester resulted in external and skeletal malformations.Exencephaly and encephalomeningocele were two of the most common external malformations,skeletal malformations consisted mainly of the incompletion of supraoccipital ossification,sternal malformation and rib malformation.FA supplementation by three ways all attenuated the external and skeletal malformations.And the best protective effect was by oral administration.The incidence of external,sternal and rib malformation was all decreased.Conclusions Three ways can prevent the mice during the second trimester from the external and skeletal malformations caused by LPS injection,and the best protective effect was by intragastric administration.
