Effect of overexpression of asporin on regulations of TGF- β 1 and Col2α1 in L929 cells
10.3969/j.issn.1006-5725.2014.16.006
- VernacularTitle:高表达asporin对L929细胞中TGF-β1及Col2α1的影响
- Author:
Hao ZHAO
;
Xinzhi LI
- Publication Type:Journal Article
- Keywords:
IDD;
Asporin;
TGF-β1;
L929
- From:
The Journal of Practical Medicine
2014;(16):2540-2543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the L929 cell line with high expression of asporin ,and to study the effect of asporin on the expressions of TGF- β1 and Col2α1 in mouse fibroblast L929. Methods Asporin cDNA was amplified from mouse mandible tissue by RT-PCR,then was inserted into the eukaryotic expression plasmid pcDNA3.1(-). The recombinant plasmid pcDNA3.1(-)-ASPNIII was transfected into the mouse fibroblast cell line L929, followed by G418 treatment. RT-PCR and Western blot assays were used to screen the stable, asporin high expressing L929 cell line.The effect of overexpression of asporin on the regulations of TGF-β1 and Col2α1 was further studied. Results The stable, aspori high expressing n L929 cell linewas successfully established. Results of RT-PCR and Western blot assays showed that overexpression of asporin could effectively inhibit the expressions of TGF-β1 and Col2α1. Conclusion Enforced expression of asporin can inhibit the expression of TGF-β1 and Col2α1 in L929 cells.
