Experimental research of combined hepatocyte growth factor with epidermal growth factor cultured human gallbladder epithelial cell
10.3969/j.issn.1671-8348.2014.22.026
- VernacularTitle:肝细胞生长因子与表皮生长因子联合培养人胆囊上皮细胞的研究
- Author:
Shikang DENG
;
Jun YUAN
;
Lianmin WANG
;
Tao WANG
;
Hao ZOU
;
Xiaowen ZHANG
- Publication Type:Journal Article
- Keywords:
bile duct;
pithelial cells;
hepatocyte growth factor;
epidermal growth factor
- From:
Chongqing Medicine
2014;(22):2903-2906
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of combined hepatocyte growth factor (HGF) with epidermal growth factor (EGF) cultured human gallbladder epithelial cells(HGBECs) in vitro .Methods The epithelial layer was peeled away from human gallbladder ,epithelial layer were digested with collagenase Ⅳ and scraped repeatedly .HGBECs were isolated and seeded in cell cul-ture plates containing medium supplemented with or without 10 ng/mL EGF or with 10 ng/mL HGF and 10 ng/mL EGF respec-tively .Then the morphologic changes of the cells were observed and taken photos with inverted phase contrast microscope ,and counted number of cells ,MTT assay detected vigor of cells in different groups .Results The number of the HGBECs of the HGF+EGF group was obviously more than the EGF group ,the duration of the HGBECs of the HGF+ EGF group was obviously longer than the EGF group(19 .3 ± 2 .5)d vs .(14 .2 ± 2 .4)d ,P< 0 .05 .And the HGBECs of the group with HGF+ EGF had better cell vigor .Conclusion HGF combines with EGF added to medium can obviously promote the proliferation of HGBECs and prolong the duration and stabilize morphology of HGBECs in vitro .
