Evaluation and improvement of anti-interference performance of D-3-hydroxybutyrate detection reagent
10.3969/j.issn.1673-4130.2014.15.010
- VernacularTitle:D-3-羟丁酸检测试剂抗干扰性能评价及其改进
- Author:
Jie WANG
;
Wenxue JIAO
;
Guichun ZHANG
- Publication Type:Journal Article
- Keywords:
interference;
D-3-Hydroxybutyrate;
vitamin C oxidase
- From:
International Journal of Laboratory Medicine
2014;(15):1991-1992
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anti-interference performance of D-3-hydroxybutyrate (D3H ) reagent kit by using the NCCLS document EP7-A2 and to improve its anti-interference performance .Methods The anti-interference evaluation test of D3H kit was performed according to NCCLS document EP7-A2 and its anti-interference performance was improved by adding vitamin C oxidase .Results 342 mol/L free bilirubin ,342 mol/L conjugated bilirubin ,1 450 FTU chyle and 5 g/L hematoglobin had no inter-ference on the high concentration D3H detection ,0 .03 g/L vitamin C interfered with the low and high concentration D3H detection . The dosage-effect experiment results showed that vitamin C produced the linear positive interference effect on D 3H detection . Adding 2 500 U/L of vitamin C oxidase could antagonize the interference of vitamin C on detection .Conclusion Vitamin C can in-terfere with the D3H detection and adding vitamin C oxidase in reagent can antagonize its interference .
