Experimental study on infertility related antibody detection in female infertility
10.3969/j.issn.1673-4130.2014.15.030
- VernacularTitle:不孕相关抗体检测女性不孕症的实验研究
- Author:
Shiying DENG
;
Jiang SHI
;
Hong NIE
- Publication Type:Journal Article
- Keywords:
infertility;
autoimmune antibodies;
protein microarray technology
- From:
International Journal of Laboratory Medicine
2014;(15):2037-2039
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether the correlation exists between 6 kinds of autoimmune antibodies and female infertility . Methods The protein microarray technique was adopted to detect serum antibodies of anti-sperm antibodies(ASAb) ,anti-endome-trial(AEmAb) ,anti-zona pellucida(AZpAb) ,anti-ovarian(AVoAb) ,anti-hCG(AhCG Ab) and anti-trophoblastic(ATAb) in 140 ca-ses of female infertility and the correlation between these 6 kinds of antibody with female infertility was evaluated .Results Com-pared with the control group ,except AvoAb ,the positive rates of other 5 kinds of antibody had statistically significant differences (P<0 .05) .In the infertility group ,the positive rate of the combined detection of these 6 kinds of autoimmune antibody had statisti-cal difference compared with that of single item detection (P<0 .05) .The positive rate of these 6 kinds of autoimmune antibody in the primary infertility group and the secondary infertility group had statistical difference compared with the control group ;the posi-tive rate of these 6 kinds of autoimmune antibody in the secondary infertility caused by different etiologies had no statistical differ-ence (P>0 .05) .The positive rate of ANA had no statistical difference between the infertility group and the control group (P>0 .05) ,but the titers of ANA in the experimenter were ≥1∶100(6/7) and most of karyotypes were nuclear coarse (5/7) ,which was differed from the low positive titer in the control group .The positive rate of ANA had statistical difference between the infertility related antibody positive and the infertility related antibody negative in the infertility patients .Conclusion ASAb ,AZpAb AEmAb , AhCGAb and ATAb are related with female sterility .The combined detection of these 6 kinds of antibody can significantly increase the detection sensitivity .
