Effect of tetrabutyl propanediamine on human acute myeloid leukemia cell line HL-60
10.3969/j.issn.1006-5725.2014.16.002
- VernacularTitle:多胺类似物四丁基丙二胺对人早幼粒白血病细胞HL-60生长的影响
- Author:
Qing WANG
;
Kai WANG
;
Yanlin WANG
;
Jianlin YANG
;
Chunyu CAO
- Publication Type:Journal Article
- Keywords:
Acute promyelocytic leukemia;
Tetrabutyl propanediamine;
Polyamine analogue;
Apoptosis
- From:
The Journal of Practical Medicine
2014;(16):2528-2531
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the new polyamine analogue tetrabutyl propanediamine (TBP) on cell proliferation and the underlying mechanism. Methods MTT assay was performed to determine cell proliferation. Flow cytometry was performed to detect cell cycle transition. DNA fragmentation and mitochondrial membrane potential determinations were performed to detect cell apoptosis. The activity of key enzymes in polyamine catabolism was detected by chemiluminescence assay. Results TBP could significantly inhibit the proliferation of HL-60 cells by blockingcell cycle transition and by inducing apoptosis. The TBP-induced apoptosis of HL-60 cells was in a dose-dependent manner. The enzyme activities of SMO and APAO were also significantly increased in HL-60 cells after treatment with 100 μM TBP for 24 hours. Conclusions TBP, as a new putrescine analogue, could inhibit proliferation of HL-60 cells by increasing the enzyme activity of SMO and APAO and inducing apoptosis.
