Anti-apoptosis effect of lipoic acid-niacin diad on acrolein-induced apoptosis in ARPE-19 cells
10.3969/j.issn.1006-5725.2014.15.009
- VernacularTitle:硫辛酸烟酸二联体拮抗丙烯醛诱导ARPE-19细胞凋亡的作用机制
- Author:
Xiulan ZOU
;
Guanfeng WANG
;
Wenli LI
;
Rongbiao PI
;
Yongzhen YU
;
Yuping ZOU
- Publication Type:Journal Article
- Keywords:
Acrolein;
ARPE-19 cell;
Lipoic acid-niacin diad;
Bax;
Bcl-2
- From:
The Journal of Practical Medicine
2014;(15):2381-2383
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulations of Bax , Bcl-2 in the protection of lipoic acid-niacin diad in acrolein-induced apoptosis in ARPE-19 cells. Methods The ARPE-19 cells were cultured in medium containing 10% fetal bovine serum , at 37 ℃ with 5% CO2. The ARPE-19 was transferred to 6-well plate after reaching to 70% confluence. After starvation for 24 h , the cells in 6-well plates were divided into three groups , including the blank control group , the acrolein treatment group with 50 μmol/L acrolein for 24 h , and the protection group with 100 μmol/L lipoic acid-niacin diad for 24 h and with the acrolein for another 24 h. The apoptotic cells were detected by flow cytometry assay , and expressions of Bcl-2 , Bax protein were detected by Western Blot assay. Results The percentages of normal healthy cells were 94.8%, 60.98%, and 91.34% in the blank control group , 50 μmol/L acrolein group and 100 μmol/L diad contained of lipoic acid and niacin group , respectively. The ratios of Bax/Bcl-2 protein expression were 0.293 9, 1.389 2, and 0.555 8 in the blank control group, 50 μmol/L acrolein group and 100 μmol/L diad contained of lipoic acid and niacin group, respectively. Conclusion The protective effect of lipoic acid-niacin diad on acrolein-induced apoptosis in ARPE-19 cell through promoting Bcl-2 expression and inhibiting Bax expression.
