Effect of recombinant human granulocyte/macrophage colonystimulating factor combined with nano-silver for deep burn degreen Ⅱ about treatment
10.3969/j.issn.1006-5725.2014.15.011
- VernacularTitle:重组人粒细胞巨噬细胞集落刺激因子联合纳米银对深Ⅱ°烫伤治疗作用的影响
- Author:
Jingzhe YANG
;
Fengping CHEN
;
Xinshu FENG
;
Hailing WEN
;
Qiying GENG
- Publication Type:Journal Article
- Keywords:
Burns;
Recombinant human granulocyte-macrophage colony stimulating factor;
Nano-silver;
VEGF;
EGF
- From:
The Journal of Practical Medicine
2014;(15):2387-2390
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of recombinant human granulocyte/macrophage colonystimulating factor (rhGM-CSF) combined with nano-silver for deep burn degreen Ⅱ. Methods The burn model were done with Wistar rats. They were randomly divided into four groups , group A (n = 30): petrolatum treatment, group B(n = 30): nano-silver treatment, group C(n = 30): rhGM-CSF treatment, and group D(n =30): rhGM-CSF combined with nano-silver treatment. The healing rates of the four groups were observed on postburn day 1, 4, 7, 10, 14, 21. Meanwhile the levels of VEGF and EGF in serums were measured with ELISA. Results All groups started to heal on postburn day 10. Group A had inflammation obviously , and group D moderately. There were significant difference in the healing retes on postburn day 10 , 14, 21 between four groups (P < 0.05). The level of VEGF in group A peaked on postburn day 21 (25.76 ± 1.46)pg/mL, but the levels of VEGF in group B, group C and group D peaked on postburn day 14[(29.73 ± 1.58)pg/mL, (38.91 ± 2.38)pg/mL, (43.54 ± 1.28)pg/mL]. On postburn day 4, 7, 10, 14, 21, there were significant difference(P <0.05). The level of EGF peaked on postburn day 21 in all groups [(0.72 ± 0.14)ng/mL, (0.93 ± 0.13)ng/mL, (1.18 ± 0.16)ng/mL, (1.50 ± 0.15)ng/mL]. There were significant difference on postburn day 7, 10, 14, 21 between four groups (P < 0.05). Conclusions rhGM-CSF combined with nano-silver treatment could promote wound healing, and be better than rhGM-CSF and nano-silver singly.
