Use ITS gene sequence analysis to identify dematiaceous fungi
10.3969/j.issn.1006-5725.2014.15.050
- VernacularTitle:ITS基因序列分析在鉴定暗色真菌中的应用
- Author:
Weizheng ZHANG
;
Qian XIAO
;
Pinghua QU
;
Guangyuan DENG
;
Song LI
;
Cha CHEN
- Publication Type:Journal Article
- Keywords:
Dematiaceous fungi;
ITS gene sequence;
PCR
- From:
The Journal of Practical Medicine
2014;(15):2493-2496
- CountryChina
- Language:Chinese
-
Abstract:
Objectives Use ITS gene sequence analysis to identify 15 strains of dematiaceous fungi , to learn the types of pathogenic strains and clinical treatment. Methods By observing the colony morphology and microscope morphological of the dematiaceous fungi isolated from superficial mycoses , and identified by ITS gene sequence analysis. Results 15 strains were identified by morphological observation as dematiaceous fungi.The amplified bands were identified by Tanon-3500 gel imaging system between 500 ~ 700 bp. Blast sequencing results show that 2 strains Alternaria alternate , 2 strains Cladosporium sphaerospermum. 2 strains Exophiala dermatitis, 1 strains Cladosporium cladosporioides, Curvularia lunata, Talaromyces rugulosus, Phaeobotryon cupressi, Cladosporium tenuissimum, Fonseceea pedrosoi, Exophiala werneckii, Exophiala oligosperma and Fonsecaea monophora. Conclusion ITS gene sequence analysis can identify dematiaceous fungi effectively , avoided undetected and misdiagnose cause by the lack of clinical experience.
