Analysis of hepatitis B virus genotyping and variation of drug resistance in 90 cases in Shenyang
10.3969/j.issn.1673-4130.2014.15.018
- VernacularTitle:沈阳地区90例乙型肝炎病毒基因分型及耐药变异分析
- Author:
Langjin JIANG
;
Jinling LI
- Publication Type:Journal Article
- Keywords:
hepatitis B;
gene chip;
genotype;
drug resistance mutation
- From:
International Journal of Laboratory Medicine
2014;(15):2008-2010
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution characteristics of hepatitis B virus (HBV) genotypes and the variation situ-ation of drug resistance gene in Shenyang area by the gene chip technology .Methods Serum samples were randomly collected from 90 inpatients of hepatitis B in 202 Hospital of PLA from August 2012 to July 2013 .The HBV genotypes and drug resistance associ-ated 5 sites in common 3 types of antiviral drug were detected by the gene chip technology of nested PCR (NPCR) combined with reverse dot blot(RDB) .Results Among 90 samples ,HBV genotypes included type B 6 .7% (6/90) ,type C 86 .6% (78/90) ,mixed type B and C 6 .7% (6/90) .The serum HBV DNA level in the patients with HBV-C genotype were significantly higher than that with genotype HBV-B and mixed genotype B and C .There were 4 cases of drug resistance gene mutation in the detected samples ,3 cases of mutation were placed in 180M+204V ,1 case of mutation was placed in 180M+204I .All the drug resistance mutations ap-peared in the patients with HBV-C genotype .Conclusion The main HBV genotype is the genotype C in Shenyang area .Genotype B and genotype mixed B and C are unusual .The HBV DNA level in the patients with the genotype C is relatively high ,which is more likely to happen resistance to lamivudine .Moreover the mixed mutation in 180M is more likely to happen .
