Analysis epidemiological characteristics of influenza virus isolated and identified cell in Huainan of Anhui Province from 2012-2013
10.3760/cma.j.issn.1008-6706.2014.17.005
- VernacularTitle:安徽省淮南市2012-2013年流感病毒细胞分离及其流行特征分析
- Author:
Shoujie YU
;
Xiaobo WANG
;
Jiang LIU
;
Xiaomei LIU
- Publication Type:Journal Article
- Keywords:
Influenzavirus A;
Influenzavirus B;
Influenzavirus C;
Cell Culture Techniques;
Hemagglutination Tests
- From:
Chinese Journal of Primary Medicine and Pharmacy
2014;(17):2573-2575
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reveal the epidemic characteristics of influenza in Huainan of Anhui Province and provide scientific basis for prevention and control of influenza surveillance by summarizing the work of influenza virus isolation performed in 2012-2013.Methods First nucleic acid fast typing by real-time PCR method for sentinel hospitals in throat swab specimens for inspection from sentinel hospital ,the influenza virus isolation was performed by using MDCK cells and the identification of the isolates was carried out by hemagglutination ( HA) and hemagglutination inhibition(HI) tests.Results 68 strains of influenza virus were isolated (45.9%) from 148 of positive specimens detected by fluorescent quantitative real-time PCR.The number of subtype A(H1N1),A(H3N2),BY and BV were 26 strains,25 strains,4 strains and 13 strains,respectively,the success rate of the separation were 56.5%,33.3%, 63.0%,respectively.There were significant difference among the separation of the strain rate of success (P<0.05). 2012 epidemic strain in Huainan was influenza BV virus ,and reached epidemic peak in March;2013 epidemic strain was A(H1N1)influenza virus,the epidemic peak was December.Conclusion During the period of influenza surveillance in Huainan,the main subtype of influenza of the year 2012 is BV which reached epidemic peak in March , while subtype A(H1N1) is the main subtype of the year 2013,with the epidemic peak month December .
