Effects of CD46-siRNA silencing on proliferation and metastasis of MCF-7 human breast cancer cell
10.3969/j.issn.1005-6483.2014.08.009
- VernacularTitle:siRNA 沉默 CD46基因表达对乳腺癌 MCF-7细胞增殖及转移能力的影响
- Author:
Jie LI
;
Jing LI
- Publication Type:Journal Article
- Keywords:
breast cancer;
CD46;
proliferation;
invasion;
migration
- From:
Journal of Clinical Surgery
2014;(8):564-567
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and observe the effects of CD46-siRNA on proliferation,in-vasion and metastasis of MCF-7 human breast cancer cell.Methods The pEGFP-C1-CD46 siRNA was established and transfected into the MCF-7 cell.Changes in CD46 mRNA and protein expression were test-ed and variation of invasion and migration capacity in MCF-7 /CD46-siRNA cell was also observed.Results Compared with the control group and scrambled group,the expression of CD46 mRNA was significantly decreased(P <0.05)and CD46 protein expression was also suppressed in the MCF-7 cells of the silence group.Proliferation activity of MCF-7 cell in the control group and scrambled group was gradually in-creased with the time,but this phenomenon was not obvious in the silence group.At the 3th and 4th day af-ter transcription,differences in proliferation activity were significant among the groups,and activity in the silent group was significantly lower than the other two groups(P <0.05).Counts in invasion and migration of MCF-7 cell in the control group and scrambled group were significantly higher than the silent group (P <0.05 ).Conclusion The expression vector of pEGFP-C1-CD46 siRNA can suppress the CD46 mRNA and protein expression in MCF-7 human breast cancer cell and reduce the capacity in proliferation activity,invasion and migration.
