Effects of penehyclidine hydrochloride on lipopolysaccharide-induced neonatal cardiomyocyte dam-age and NF-κB activation
10.3969/j.issn.1005-6483.2014.08.024
- VernacularTitle:盐酸戊乙奎醚对脂多糖诱导新生乳鼠心肌细胞损伤及 NF-κB 活化的影响
- Author:
Lingjie ZHANG
;
Junfeng ZHANG
;
Tao WANG
- Publication Type:Journal Article
- Keywords:
penehyclidine hydrochloride;
cardiomyocyte;
lipopolysaccharide;
NF-κB
- From:
Journal of Clinical Surgery
2014;(8):603-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of penehyclidine hydrochloride(PHC)on lipopo-lysaccharide-induced neonatal cardiomyocyte damage.Methods After the primary cardiomyocytes culture of neonatal rat on postnatal day 1,the myocytes were randomly divided into 4 groups:the control group,the lipopolysaccharide(LPS)group,the low dosage PHC pretreatment group and high dosage PHC pretreatment group.Activities of myocardial enzymes and cardiac troponin and cell survival rate were measured.Expres-sion of NF-Κb(p65)and IκB-α(NF-κB inhibitor)phosphorylation were assessed using Western blot.Re-sults Compared with the control group,activities of myocardial enzymes and cardiac troponin in the LPS group were elevated but the survival rate were down-regulated.All these effects were reversed in the PHC pretreatment group.Results of Western blot showed that phosphorylation of IκB-αand p65 expression were up-regulated in the LPS group.In the PHC pretreatment group,phosphorylation of IκB-αand p65 expres-sion were down-regulated.Conclusion Penehyclidine hydrochloride can inhibit lipopolysaccharide-in-duced neonatal cardiomyocyte damage and the down-regulation of NF-κB activity might be involved.
