Prokaryotic expression of NrdF1, PE_PGRS35, Rv1985c and Rv1986 of Mycobacterium tuberculosis and the potential application for the detection of antibodies in bovine tuberculosis

VernacularTitle:结核分枝杆菌NrdF1、PE_PGRS35、Rv1985c和Rv1986的原核表达及检测牛结核病抗体之应用
Author: Lin SUN ; Yan LIU ; Chenyu MIN ; Yachen HU ; Xiang CHEN ; Xinan JIAO
Publication Type:Journal Article
Keywords: Mycobacterium tuberculosis; NrdF1; PE_PGRS35; Rv1985c; Rv1986; bovine tuberculosis; serodiagnosis
From: Chinese Journal of Zoonoses 2014;(8):782-786
CountryChina
Language:Chinese
Abstract: Proteins encoded by regions of difference (RD) of Mycobacterium tuberculosis (MTB) constitute a potential source of specific antigens for vaccine development and immunodiagnosis .In the present study ,four genes named nrdF1 , pe_pgrs35 ,rv1985c ,and rv1986 from RD2 of MTB were cloned and overexpressed in E .coli with the induction of IPTG .Western blotting assay showed that these recombinant fusion proteins could well react with anti-His tag monoclonal antibody ,which in-dicated their good immunoreactivity .The serodiagnosis potential applications of these four proteins in bovine tuberculosis were further evaluated .An indirect ELISA assay was established by using fusion proteins as coating antigens for detection of their specific antibodies in bovine sera .The positive rates were 7 .35% in NrdF1 ,22 .06% in PE_PGRS35 ,16 .18% in Rv1985c and 16 .18% in Rv1986 respectively .All the results suggest that these fusion proteins have the potential application in serodiagnosis of bovine tuberculosis .