Isolation,identification and full-length genome sequence of EMCV f rom domesticated boar

VernacularTitle:家养野猪脑心肌炎病毒的分离、鉴定及全基因组序列分析
Author: Hongtao CHANG ; Huimin LIU ; Jun ZHAO ; Lu CHEN ; Xia YANG ; Xinwei WANG ; Huixia YAO ; Chuanqing WANG
Publication Type:Journal Article
Keywords: encephalomyocarditis virus; domesticated boar; RT-PCR; full-length genome; molecular analysis
From: Chinese Journal of Zoonoses 2014;(8):793-796
CountryChina
Language:Chinese
Abstract: Encephalomyocarditis virus (EMCV) ,named JZ1202 ,was isolated from domesticated boar in Henan ,China . We performed the full-length genome sequencing and molecular characteristic analysis of the isolated strain .Results showed that the full-genome sequence of EMCV JZ1202 generated a sequence of 7 735 bp in length ,and had 81 .2%-99 .9% nucleotide identity with other reference strains from different animals ,but 99 .4% with Chinese reference from pig .The phylogenetic tree was constructed based on the full-length genome;ORF and VP1 gene sequences identified EMCV was divided into G1 ,G2 and G3 groups ;the strain JZ1202 belongs to G1 with other Chinese reference strains .Results identified that the EMCV infection could cause severe clinical symptoms in domesticated boar .Big regional differences exist in EMCV and the transmission is limit-ed in a range of area .However ,cross infection and prevalence of EMCV disease between domesticated boar and mice might ex-ist .Mutation of some amino acid may occurred in EMCV infected domesticated boar .