Spatio-temporal expression of P2X3 receptor in rats with diabetic mechanical allodynia
10.3969/j.issn.0529-1356.2014.04.018
- VernacularTitle:糖尿病机械性痛模型大鼠 P2 X3受体的时空表达
- Author:
Yuanyuan CUI
;
Huanghui WU
;
Lan WANG
;
Juan SHI
;
Yunqing LI
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Mechanical allodynia;
P2X3 receptor;
Spinal dorsal horn;
Dorsal root ganglion;
Immunofluorescence;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2014;(4):540-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the spatio-temporal expression of P2X3 receptor ( P2X3R) in rats with diabetic mechanical allodynia ( DMA ) .Methods DMA model in rats was induced by intraperitoneal injection of streptozocin ( STZ) .The von Frey filaments were applied to identify the changes of the paw withdrawal threshold ( PWT) in DMA rats.Immunofluorescence was employed to detect the spatio-temporal expression of P2X3R in the spinal dorsal horn (SDH), dorsal root ganglion (DRG) and hind paw skin on different time points after intraperitoneal injection of STZ , respectively.The protein expression of P2X3R in SDH and DRG was further semi-quantitatively analyzed by Western blotting.Results Compared with control group , intraperitoneal injection STZ induced significant mechanical allodynia indicated by the reduced PWT from 7 days, and which reached the peak on 14d and maintained to 28days (P<0.05). The expression of P2X3R in DRG neurons was significantly increased on 14 days and 21 days (P<0.05), while that in SDH and skin was markedly increased on 21 days and 28 days, compared with control group (P<0.05).Conclusion With the progress of DMA, the expression of P2X3R was significantly increased in the SDH, DRG and skin, which was almost parallel with the mechanical allodynia , but the changes in SDH and skin were 1 week later than that in DRG .These results suggest that P2X3R may play a key role in the maintenance of the DMA .
