Application of Cell Counting Kit-8 in detecting the growth inhibiting effect of 5-Aza-2 ’-deoxycytidine on chronic myeloid leukemia cells
10.3969/j.issn.0529-1356.2014.04.026
- VernacularTitle:活细胞计数试剂盒在检测5-氮杂-2’-脱氧胞苷对慢性粒细胞白血病细胞的增殖抑制作用中的应用
- Author:
Yue LIU
;
Chun SHUAI
;
Jiesheng LI
;
Hong YIN
;
Yanbin SONG
;
Wenli MA
- Publication Type:Journal Article
- Keywords:
Cell Counting Kit-8;
5-Aza-2’-deoxycytidine;
K562;
Proliferation
- From:
Acta Anatomica Sinica
2014;(4):582-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the application of Cell Counting Kit-8(CCK-8) in detecting the growth inhibiting effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cell .Methods The proliferation of K562 cells was detected by CCK-8 with different concentrations of 5-Aza-2 ’-deoxycytidine and the cell cycle and apoptosis of K 562 cells were detected after K562 treated by 50% inhibitory concentration of 5-Aza-2 ’-deoxycytidine .Results The 50% inhibitory concentration of 5-Aza-2’-deoxycytidine was 15.55nmol/L, after treated with this concentration , K562 cells showed that G2 phase arrest occurred , proliferation inhibited and apoptosis peaks appeared .Conclusion Inhibition of proliferation of K562 cells with different concentrations of 5-Aza-2’-deoxycytidine varied in a dose-dependent relationship , and 5-Aza-2’-deoxycytidine could promote apoptosis of K 562 cells.CCK-8 can be used in detecting the effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cells .
