MicroRNA-122 expression during isoniazid anti-tuberculosis drug-induced liver injury in mice
10.3867/j.issn.1000-3002.2014.04.015
- VernacularTitle:微小 RNA-122在异烟肼所致小鼠抗结核药物性肝损伤中的动态变化
- Author:
Lei SONG
;
Zhongrui ZHANG
;
Lei HE
;
Li GAO
;
Zhe SHI
;
Fumin FENG
- Publication Type:Journal Article
- Keywords:
microRNA-122;
isoniazid;
anti-tubercuIosis drug;
Iiver injury;
oxidative damage;
bioIogicaI factors
- From:
Chinese Journal of Pharmacology and Toxicology
2014;(4):569-574
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTlVE To study the reIationship between microRNA(miRNA)-122 and Iiver injury in-duced by anti-tubercuIosis drugs,and to discover the new biomarkers for earIy diagnosis of this type of Iiver injury. METHODS mice were given 2 mL isoniazid oraIIy at 90 mg·kg-1 . BIood and Iiver tissue sampIes were coIIected at 1,3,5,7,14,21 and 28 d after administration of isoniazid. Serum gIutamic-pyruvic transaminase( GPT)and gIutamic-oxaIoacetic transaminase( GOT)IeveIs were determined using an automatic biochemicaI anaIyzer. Cu/ Zn-superoxide dismutase( Cu/ Zn-SOD ) activity and maIondiaIdehyde( mDA)content were detected by biochemicaI method. ReaI-time qPCR was used to measure the expression of miRNA-122. RESULTS GPT and GOT IeveIs were significantIy higher at 14 and 21 d(P﹤0.05)than in the controI. Cu/ Zn-SOD began to decIine whiIe mDA began to increase after 5 d(P﹤0.05). miRNA-122,which progressiveIy decreased after administration,was reduced to the mini-mum 0.58 ±0.02 at 14 d. There were good correIations between miRNA-122 and GPT,Cu/ Zn-SOD, mDA(the correIation coefficients were -0.370,0.268,and -0.298,respectiveIy),but no correIation with GOT was observed. CONCLUSlON The tissue miRNA-122 profiIe can be used as a sensitive marker for anti-tubercuIosis drug-induced Iiver injury,which couId contribute to the earIy diagnosis of Iiver injury.
