Resistance mechanism and molecular epidemiological characteristics of isolated clinically carbapenem-resistant Acinetobacter baumannii in Meizhou, China
10.3969/cjz.j.issn.1002-2694.2014.08.009
- VernacularTitle:梅州地区临床分离耐碳氢霉烯类鲍曼不动杆菌的耐药机制及分子流行病学研究
- Author:
Guangwen XIAO
;
Xuetao WANG
;
Yafeng QIAO
;
Shangping ZOU
;
Zhendong YE
- Publication Type:Journal Article
- Keywords:
A cinetobacter baumannii;
carbapenemase;
modified Hodge test;
Beta lactamase
- From:
Chinese Journal of Zoonoses
2014;(8):816-820
- CountryChina
- Language:Chinese
-
Abstract:
In order to survey antibiotic resistance of clinical isolates carbapenem-resistant A cinetobacter baumannii in Meizhou and to investigate resistance mechanism and molecular epidemiological characteristics ,a total of 210 non-duplicated clinical isolates of carbapenem-resistant Acinetobacter baumannii from January 2012 to December 2012 were collected .The K-B disk diffusion method was applied for the drug-susceptibility test ,a modified Hodge test was used for the screening of carbapen-emase ,PCR was used to amplify carbapenemase genes (including IMP ,VIM ,OXA-23 ,OXA-24 ,OXA-51 and OXA-58) ,and the positive products were sequenced .Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) was used for DNA typing and test of homology .Our results on the percentage of strains resistant for antibiotics tested were higher than 60% except for polymyxin B was 0 .48% .There were 163 positive strains by the modified Hodge test ,accounting for 77 .62% .OXA-51 gene was identified in 198 strains (94 .29% ) ,OXA-23 in 165 strains (78 .57% ) ,and VIM in 9 strains (4 .29% ) ,OXA-24 ,OXA-58 and IMP gene was not identified by PCR amplification .Seven genomic types were included in the 210 carbapenem-resistant Acinetobacter baumannii .The major prevalence types were Type A (97 strains) ,Type B (44 strains) and Type H (25 strains) . In conclusion ,multiple drug resistance of clinically isolated carbapenem-resistant A cinetobacter baumannii is a serious problem in Meizhou .Production of OXA-51 ,OXA-23 and IMP carbapenemases is an important mechanism of resistance to carbapenem antibiotics ,and there is prevalence of the same clones in these carbapenem-resistant strains .
