Expression of Caspase-12 in cellular apoptosis of hippocampus in the rat model of posttraumatic stress disorder
10.3969/j.issn.0529-1356.2014.04.003
- VernacularTitle:创伤后应激障碍大鼠海马神经元凋亡中Caspase-12的表达
- Author:
Hong LIU
;
Fang HAN
;
Yuxiu SHI
- Publication Type:Journal Article
- Keywords:
Posttraumatic stress disorder;
Hippocampus;
Neuron;
Wstern blotting;
Rat
- From:
Acta Anatomica Sinica
2014;(4):452-456
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe changes of apoptosis and Caspase-12 expression in hippocampus of the rat model of posttraumatic stress disorder ( PTSD ) .Methods Single prolonged stress ( SPS ) was used to duplicate a rat model of PTSD.Male Wistar rats were randomly divided into 1, 4, 7 days groups after exposure to SPS and a normal control group.Transmission electron microscopy was used to observe morphologic changes of the hippocampus neurons .The expression of Caspase-12 was detected by immunohistochemistry , Western blotting and RT-PCR.Results After SPS, the hippocampus neurons had characteristic morphologic changes of apoptosis , and the expression of Caspase-12 reached the peak at SPS 4d.Conclusion PTSD activates the ERS-mediated apoptosis in hippocampus via Caspase-12, which may be a pathophysiology base of the atrophy of the hippocampus in PTSD .
