Effect of uncoupling protein 2 on activation of hepatic stellate cell in liver fibrosis of rats
10.3969/j.issn.0529-1356.2014.04.008
- VernacularTitle:解耦联蛋白2对大鼠肝纤维化形成中星形细胞活化的影响
- Author:
Jianduo AN
;
Ying JIANG
;
Yunfei BAI
;
Xuejiang WANG
- Publication Type:Journal Article
- Keywords:
Uncoupling protein 2;
Liver fibrosis;
Hepatic stellate cells;
p38 Mitogen activated protein kinase;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2014;(4):480-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of uncoupling protein 2 ( UCP2) in the development of hepatic fibrosis and its molecular mechanism .Methods The CCl4-induced liver fibrosis rat model in vivo was established to observe the pathological changes of rat livers .The expression levels of UCP2 and p38 mitogen activated protein kinase (p38 MAPK) were detected by using the techniques of Western blotting , Real-time PCR and immunohistochemistry .The hepatic stellate cells ( HSC) were stimulated by CCl 4 and UCP2-specific inhibitor Genipin to mimic liver fibrosis in vitro.The expression levels of UCP2 and p38MAPK were determined by using Western blotting .Results We found that UCP2 and α-SMA expression levels increased significantly (P <0.05, n =10) in the liver of rats with CCl4-induced liver fibrosis when compared with that of the normal control rats in vivo.Similarly, the expression levels of UCP2 and p38 MAPK were up regulated (P <0.05, n=6) in CCl4-treated HSC cells in vitro.However, the expressions of UCP2 and p38 MAPK were down regulated (P <0.05, n=6) in genipin-treated HSC cells in vitro.Conclusion UCP2 is involved in liver fibrosis, and probably contributed to the activation and proliferation of hepatic stellate cells .
