5-Aza-2 ’-deoxycytidine and 4-phenylbutyric acid exert a cooperative effect on the expression of miR-196 b in chronic myeloid leukemia cells
10.3969/j.issn.0529-1356.2014.04.014
- VernacularTitle:5-氮杂-2’-脱氧胞苷和4-苯基丁酸对慢性粒细胞白血病细胞的miR-196b表达水平有协同作用
- Author:
Yue LIU
;
Chun SHUAI
;
Jiesheng LI
;
Hong YIN
;
Yanbin SONG
;
Wenli MA
- Publication Type:Journal Article
- Keywords:
miR-196b;
Methylation;
Histone acetylation;
K562cell;
Real-time PCR;
Human
- From:
Acta Anatomica Sinica
2014;(4):521-524
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study if 5-Aza-2’-deoxycytidine along or together with 4-phenylbutyric acid could affect miR-196b expression levels in chronic myeloid leukemia cells .Methods K562 cells were treated with DNA methylation inhibitor 5-Aza-2’-deoxycytidine, histone deacetylase inhibitors 4-phenylbutyric acid separately and the combined treatment with both of them, then expression levels of miR-196b were detected using Real-time PCR.Results The half inhibition concentration of 4-phenylbutyric acid was 1.58mmol/L.Comparing with the expression level of miR-196b in normal human bone marrow cells, the expression levels of miR-196b were significantly lower in Aza group , PBA group and negative control cells and nearly consistent among three groups , and as high as normal cells in combined treatment group . Conclusion The expression level of miR-196b in K562 cells could not return to normal treated with 5-Aza-2 ’-deoxycytidine or 4-phenylbutyric acid separately , while could restore normal when treated with both agents , indicating that miR-196b expression level in K562 cells is related with both DNA methylation and histone acetylation .
