Effects of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil on angiogenesis regulatory factors
10.3969/j.issn.0529-1356.2014.04.015
- VernacularTitle:灵芝孢子油及灵芝提取物孢子油对乳腺癌血管生成调节因子的作用
- Author:
Jing ZHANG
;
Miaomiao NIU
;
Li YANG
;
Lisha FAN
;
Li WU
;
Jun ZHAN
;
Hongquan ZHANG
- Publication Type:Journal Article
- Keywords:
Ganoderma lucidum spore oil;
Ganoderma lucidum extraction spore oil;
Epidermal growth factor receptor;
Vascular endothelial growth factor;
Thrombospondin-1;
Western blotting
- From:
Acta Anatomica Sinica
2014;(4):525-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil in neovascularization of human high malignant breast cancer .Methods Human high malignant breast cancer cell MDA-MB-231 and tumor-bearing nude mice established with MDA-MB-231 were treated with different doses of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil .Epidermal growth factor receptor ( EGFR) expression level was examined by Western blotting and the RNA expression levels of neovascularization related molecules such as EGFR , vascular endothelial growth factor (VEGF), metalloproteinases(MMPs), thrombospondin(TSP-1), platelet derived growth factor( PDGF) , fibroblast growth factor ( FGF) were detected by Real-time PCR.Results Both ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil inhibited the expression of EGFR in vitro and in vivo in a dose-dependent way.Both compounds induced down-regulation of VEGF and up-regulation of TSP-1 at RNA level.The effect of Ganoderma lucidum extraction spore oil was more significant than that of ganoderma lucidum spore oil .Conclusion Both ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil inhibite the expression of neovascularization related molecules and increase the expression of molecules inhibiting neovascularization , whereas the effect of ganoderma lucidum extraction spore oil is more obvious .
