Silence regulatory protein 1 promotes axonal outgrowth in vitro
10.3936/j.issn.1002-0152.2014.06.001
- VernacularTitle:沉默调节蛋白1促进体外神经元的轴突生长
- Author:
Haiqian LIANG
;
Xiaohong LI
;
Jingjing WANG
;
Yue TU
;
Chong CHEN
;
Sai ZHANG
- Publication Type:Journal Article
- Keywords:
Sirt1;
Axon growth;
Resveratrol;
Neuron
- From:
Chinese Journal of Nervous and Mental Diseases
2014;(6):321-324
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of silence regulatory protein 1 (Sirt1) on axonal outgrowth. Methods The hippocampal neurons was first isolated in vitro from rat embryos. The distribution and expression of Sirt1 were then detected 72 h later. The down-regulation of Sirt1 was induced by RNAi technology and up-regulation of Sirt1 was in-duced by overexpression of Sirt1 and resveratrol (RES). Immunofluorescence staining was used to examine the axon length. Results Immunofluorescence staining showed that Sirt 1 was located in neuronal cell body and neurite, especially in the distal axons. Down-regulation of Sirt1 significantly decreased axonal length compared with siRNA control group [(178.3 ± 3.2) μm vs. (110.2 ± 18.30) μm, P< 0.01 ]; Overexpression of Sirt1 significantly increased axonal length com-pared with eGFP control group [(178.3±3.2)μm vs (310.6±39.5)μm, P<0.01 ];Activation of Sirt1 by RES treatment al-so significantly increased axonal length compared with vehicle control group (DMSO treated group) [(291.7±13.2)μm vs. (525.1±49.1)μm, P<0.01 ]. Conclusions Sirt1 plays a key role in axonal growth which may be used as a potential thera-peutic target of axon regeneration.
