Metabonomics Study on Luteolin Intervention of Breast Cancer MCF-7 Cells
10.11895/j.issn.0253-3820.131230
- VernacularTitle:细胞代谢组学用于木犀草素抑制MCF-7细胞的机制研究
- Author:
Dongdong SHI
;
Guiming WANG
;
Yuanyuan KUANG
;
Zhangxiao PENG
;
Yan WANG
;
Xue GU
;
Chao YAN
- Publication Type:Journal Article
- Keywords:
Gas chromatography/mass spectrometry;
Cell metabonomics;
MCF-7 cells;
Cell cycle;
Breast cancer;
Luteolin
- From:
Chinese Journal of Analytical Chemistry
2014;(8):1088-1093
- CountryChina
- Language:Chinese
-
Abstract:
The metabolic profiles of control and MCF-7 cells treated with luteolin were analyzed separately using gas chromatography/mass spectrometry ( GC/MS ) to study the mechanism of the luteolin treatment on MCF-7 cells. Cell viability assays showed that luteolin had inhibition effect on MCF-7 cells. Partial least square discriminant analysis ( OPLS-DA) was used to process the metabolic data. Since cells in phase of S were increased significantly, we speculated that luteolin had a blocking effect on pentose phosphate pathway of MCF-7 cells, which contributed to its inhibition effect on proliferation of MCF-7 cells.
