Determination of Quinolones in Spicy Soup Using Packed Fiber Solid Phase Extraction and High Performance Liquid Chromatography-Fluorimetric Detection
10.11895/j.issn.0253-3820.140067
- VernacularTitle:纳米纤维固相萃取-高效液相色谱-荧光检测麻辣烫汤液中喹诺酮类药物
- Author:
Siwei DENG
;
Jianjun DENG
;
Tingting WANG
;
Yu WANG
;
Xuejun KANG
- Publication Type:Journal Article
- Keywords:
Spicy soup;
Quinolones;
Packed fiber solid phase extraction;
High performance liquid chromatography;
Fluorimetric detection
- From:
Chinese Journal of Analytical Chemistry
2014;(8):1172-1177
- CountryChina
- Language:Chinese
-
Abstract:
An analytical method for simultaneous determination of five quinolones in spicy soup was developed. Spicy soup samples were firstly extracted by EDTA-Mcllvaine buffer at pH 4, then purified and concentrated by a novel Packed fiber solid phase extraction ( PFSPE ) coulumn. The extracted liquid supernatant was loaded onto the column, rinsed with water, and then eluted with 2% ammoniated methanol. The mobile phase was methanol-water-phosphoric acid (25:75:0. 1, V/V, adjusting the pH to 2. 8 with triethylamine) . These analytes were quantified by high performance liquid chromatography-fluorimetric detector( HPLC-FLD) at excitation and emission wavelength of 280 nm and 450 nm respectively. Recoveries of spiked quinolone antibiotics in spicy soup were from 72 . 1% to 110 . 3% with intraday relative standard deviation (RSD) between 1. 6% and 4. 3% and inter-day RSD from 2. 0% to 4. 3%. Limit of detection (LOD) and limit of quantitation(LOQ) were from 1. 2 to 5. 4 μg/L and from 3. 9 to 18 μg/L, respectively. The method could be applied to determine the quinolones in spicy soup.
