Comparison of susceptibility testing by EUCAST and CLSI broth microdilution methods against Aspergillus isolates
- VernacularTitle:EUCAST和CLSI微量稀释法检测曲霉体外药物敏感试验差异比较
- Author:
Fei CHEN
;
Wenkui SUN
;
Ting WU
;
Wenjie YAN
;
Xin SU
;
Yi SHI
- Publication Type:Journal Article
- Keywords:
Aspergillus;
European Committee on Antimicrobial Susceptibility Testing;
Clinical and Laboratory Standards Institute;
minimum inhibitory concentration;
minimum effective concentration
- From:
Chinese Journal of Infection and Chemotherapy
2014;(4):338-343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the results of susceptibility testing by European Committee on Antimicrobial Susceptibility Testing (EUCAST)and Clinical and Laboratory Standards Institute (CLSI)broth microdilution methods against Aspergillus isolates.Methods The susceptibilities of 116 Aspergillus isolates were determined for amphotericin B, voriconazole, itraconazole,caspofungin and micafungin according to EUCAST (E.DEF 9.1 )and CLSI (M38-A2)methods.The essential agreement (EA),categorical agreement (CA),very major errors (VME)and major errors (ME)of the two methods were compared.Results The EA was 96.3%-100% between the two methods.The CA ,ME,and VME were 98.8%,0-1.2% and 0 respectively for the susceptibility of Aspergillus fumigatus to voriconazole.The CA,ME and VME was 1 00%,0 and 0 respectively for the susceptibility of Aspergillus fumigatus and Aspergillus niger to amphotericin B,or the susceptibility of Aspergillus fumigatus and Aspergillus flavus to itraconazole.Conclusions The results of susceptibility testing by EUCAST and CLSI broth microdilution methods are well consistent against Aspergillus isolates.
