Preventive effect of vitamin E on chronic liver injury induced by N,N-dimethylformamide in mice
10.3867/j.issn.1000-3002.2014.04.007
- VernacularTitle:维生素E对二甲基甲酰胺致小鼠慢性肝损伤的预防作用
- Author:
Xiumei ZHANG
;
Chao HIANG
;
Sufang YU
- Publication Type:Journal Article
- Keywords:
dimethyIformamide;
vitamin E;
Iiver damage
- From:
Chinese Journal of Pharmacology and Toxicology
2014;(4):515-518
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTlVE To study the preventive effect of vitamin E(Vit E)on chronic Iiver injury in-duced by N,N-dimethyIformamide( DmF). METHODS HeaIthy maIe Kunming mice were randomIy divided into 4 groups,10 mice in each group. The mice in DmF modeI group and DmF+Vit E group were ig given DmF 0.5 g·kg-1 ,whiIe Vit E controI group and DmF+Vit E group were ig given Vit E 5 mg·kg-1 after 2 h once a day for 30 d. The activity of serum gIutamic-oxaIoacetic transaminase(GOT),gIutamic-pyruvic transaminase(GPT)and xanthine oxidase(XOD)and the content of maIondiaIdehyde(mDA) in Iiver tissue were measured with corresponding kits. The pathoIogicaI changes of Iiver tissue were ob-served with HE staining. RESULTS Compared with the normaI controI group,the activity of serum GOT,GPT and XOD of the DmF modeI group was significantIy eIevated(P﹤0.05,P﹤0.01),and the Iiver ceII nucIeus pycnosis,ceIIuIar edema,degeneration,necrosis occurred. Compared with DmF modeI group,the activity of serum GOT,GPT and XOD of DmF+Vit E group were aII significantIy reduced(P﹤0.05,P﹤0.01),the content of mDA in Iiver tissue was significantIy diminished(P﹤0.01),but histopatho-IogicaI changes of Iiver tissue were significantIy improved. CONCLUSlON Vit E has preventive effect on chronic Iiver damage induced by DmF through antioxidation.
